New PDF release: Adult heart muscle cells: Isolation, properties and

By Dr. T. Powell (auth.), H. M. Piper, P. G. Spieckermann (eds.)

ISBN-10: 3662110415

ISBN-13: 9783662110416

ISBN-10: 3662110431

ISBN-13: 9783662110430

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Additional resources for Adult heart muscle cells: Isolation, properties and applications

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8 30 . mM 20 . 3 _5 10 . D. 4[1 ,2(' ... Fig. 2. Influence of pH on lactate uptake of isolated cardiac myocytes at two extracellular lactate concentrations: x, ± SEM, n: 3- 6. 59 In other cell types lactate transfer by monocarboxylic acid transporters is reported to depend also on extracellular pH (S, 14). This could also be demonstrated in our study. 1 i. e. S mM (Fig. 2). Discussion The results presented yield three essential findings: i. e. first the type of kinetics ofL-lactate uptake, second its dependence on pyruvate and third its dependence on pH.

100. o... o. ~ s. / / ~Jft--'I ZO. 40. 60. BO. 100. (mM) Lactate Fig. I. 3 mM pyruvate (/1). The insert shows an expended scale graph of the lower left part of the graph . The straight line just obove the dOlled zerouptake line represents the calculated non-ionic transfer. x, ± SEM , n: 3-8. 0 mM. 11. 8 30 . mM 20 . 3 _5 10 . D. 4[1 ,2(' ... Fig. 2. Influence of pH on lactate uptake of isolated cardiac myocytes at two extracellular lactate concentrations: x, ± SEM, n: 3- 6. 59 In other cell types lactate transfer by monocarboxylic acid transporters is reported to depend also on extracellular pH (S, 14).

M. Piper, P. Schwartz, R. Spahr, J. F. Hutter and P. G . ) Summary Cultured adult cardiocytes were exposed to anoxia. The initial decrease of high-energy phosphates was accompanied by a moderate release of cytosolic enzymes and morphological changes: the appearance of sarcolemmal 'micro blebs' ( ~ I 11m in diameter) and an increase of subsarcolemmal vesicles. At ATP levels above 2Ilmol/gww, metabolic and morphological alterations were reversible. Probably the sarcolemmal changes are causally related to the loss of macromolecules from reversibly injured cells.

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Adult heart muscle cells: Isolation, properties and applications by Dr. T. Powell (auth.), H. M. Piper, P. G. Spieckermann (eds.)


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