Download PDF by Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson,: A Laboratory Guide to Glycoconjugate Analysis

By Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, Prof. John T. Gallagher (eds.)

ISBN-10: 3034873883

ISBN-13: 9783034873888

ISBN-10: 3034873905

ISBN-13: 9783034873901

18. 2 precept of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. three Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. four Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. five size of Binding consistent for the interplay among Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 dimension of Binding consistent for the interplay among Protein and local Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ the appliance of Capillary Affinity Electrophoresis to the research _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 advent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 precept of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. three choice of organization Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. four Technical systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 basic concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. five barriers of the process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 program of CAE to the research of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 creation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical tactics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. three pattern Detection and pattern restoration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and marking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 pattern detection by means of blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. four research of knowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring pattern mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical research of information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 reading ACE styles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 opposite ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. five precis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 topic Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII checklist of participants Nebojsa Avdalovic John T. Gallagher Dionex company melanoma learn crusade division of scientific Oncology 445 Lakeside force collage of Manchester Sunnyvale, CA 94086 Christie CRC study Centre Klaus Biemann Wilmslow highway division of Chemistry Manchester M20 4BX Massachusetts Institute of expertise united kingdom Cambridge, MA 02139-4307 united states Geoffrey R.

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Subtilis. or the enzyme from Trypanosoma bruce; may be used. PI-PLC cleaves the GPI a~chor to generate the cross-reacting determinant (CRO). Both the recombinant form of the enzyme from B. thur;ngiens;s (Oxford GlycoSystems cat. no. GPI-02) and the native enzyme from T. bruce; (Oxford GlycoSystems cat. no. GPI-03) are commercially available. 2. Separate the cleaved product by extraction into a detergent such as Triton X-114. In this technique the uncleaved material with the lipid anchor attached will extract into the detergent phase.

2 Varki A (1993) Glycobiology 3: 97. \0 Laemmli UK (1970) Nature 277: 680. D. Hannes and D. 4 of the sugar chains of glycoproteins. Eur. Rickwood (eds) An Introduction to Polya- 1. Biochem. 209: 483. cylamide Gel Electrophoresis: A Practical Dwek RA, Edge Cl, Harvey 01, Wormald Approach, IRL, Oxford. MR and Parekh RB (1993) Glycoform analysis of glycoproteins. Ann. Rev. Biochem. 62: 6. 5 Kobata A (1992) Structures and functions of the sugar chains of glycoproteins. Eur. J. Biochem. 209: 483 . 6 7 8 12 Towbin H, Towbin R, Dunbar IS, and Bone K (1979) Proc.

Chromatogr. 436: 328 47 Davies Ml, Smith KD, Carruthers RA, 30 Neue UD (1975) unpublished results Chai W, Lawson AM and Hounsell EF 31 Brons C and Olieman C (1983) (1993) 1. Chromalogr. 646: 317 1. Chromatogr. 259: 79 32 Feste AS and Khan I (1992) 1. Chromatogr. 607: 7; Feste AS and Khan I (1993) 1. Chromatogr. 630: 129 33 Verzele M and Van Damme F (1986) 1. Chromatogr. 362: 23 34 Simms Pl, Haines RM and Hicks KB (1993) 1. Chromatogr. 648: 131 35 Bendiak B, Orr 1, Brockhausen I, Vella G and Phoebe C (1988) Anal.

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A Laboratory Guide to Glycoconjugate Analysis by Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, Prof. John T. Gallagher (eds.)


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